The first aquatic preserve established in Florida, this is one of the most productive estuaries in the state. The bay is home to a wide variety of wildlife, including the bald eagle. The preserve protects the water, inlets, and islands along 10 miles of Estero Bay. Visitors can canoe or kayak in the bay or on the Estero River. Launch facilities are available at Koreshan State Historic Site and Lovers Key/Carl E. Johnson State Park. Miles of trails offer visitors the opportunity to hike, bicycle, or study the variety of wildlife and native vegetation protected here. There are gopher tortoises, fiddler crabs, slash pines, and live oaks.
The old rail bed of the Seaboard Air Line Railway can be found near the Florida Power and Light easement headed northwest. This section has now been orphaned as developments to the north and south have isolated this section. The Seaboard Air Line Railway was once one of two railroads that served southwest Florida and serves as a point of interest in the park on a hike.